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產(chǎn)品詳情

  • 產(chǎn)品名稱：Anti-pro Caspase 3 (Pro and Active) Rabbit mAb

  • 產(chǎn)品型號：EB-AT0769
  • 產(chǎn)品廠商：CASMASCI
  • 產(chǎn)品文檔：
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簡單介紹：
Anti-pro Caspase 3 (Pro and Active) Rabbit mAb Size: 100ul Concentration: 1mg/ml
詳情介紹：

Anti-pro Caspase 3 (Pro and Active) Rabbit mAb

Background: 

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. The Caspase-3 polyclonal antisera recognizes the proform of caspase-3 (approx. 32) kDa), and the large (approx. 14-21 kDa) and small (approx. 10 kDa) subunits of active/cleaved Caspase-3.
 
UniProt ID: P42574

Overview
Reactivity
Human, Mouse, Rat, Bovine, Pig, Monkey
Tested applications
Western Blotting 1:1000
Immunofluorescence  1:500
Immunohistochemistry (Paraffin) 1:500
Immunoprecipitation(IP)  1:100 
Optimal dilutions/concentrations should be determined by the end user. 
 
Specificity
This antibody detects inactive (pro) and active (cleaved) forms of the enzymes. The presence of the large or small subunits in western blots is considered to be a marker of caspase activation.
Properties
Immunogen
Recombinant full-length human Caspase-3 protein (pro-form) was used as immunogen.
Clonality
Monoclonal, clone number: 5F4
Isotype
Rabbit IgG
Form
Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide
Storage instruction
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.
Host
Rabbit
Applications
WB Image

Western Blot: - Caspase-3 Antibody - (Pro and Active).
Lysates from Jurkat cells (lane 1), normal mammary tissue (lane 2) and surgical specimens from three invasive ductal carcinomas (lanes 3-5) were normalized for total protein content (50 ug/lane) and western blotted with anti-Caspase-3. The ~32 kDa pro-Caspase-3 protein was detected in all samples. Active/cleaved Caspase-3 was identified in Jurkat and two ductal carcinomas (14-21 kDa large subunit).


Simple Western: Caspase-3 Antibody - (Pro and Active)- Simple Western lane view shows a specific band for Caspase 3 in 0.2 mg/ml of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
 
IHC Image

Immunohistochemistry-Paraffin: Caspase-3 Antibody - (Pro and Active) - Analysis of Caspase-3 expression in formalin-fixed, paraffin-embedded human breast ductal carcinoma in situ using this antibody at 1:2000. Staining is seen in the the cancerous ducts, but not in the normal lobulus.
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