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產(chǎn)品詳情

  • 產(chǎn)品名稱：Recombinant Human Platelet Factor-4/CXCL4

  • 產(chǎn)品型號(hào)：CA20104
  • 產(chǎn)品廠商：其它品牌
  • 產(chǎn)品文檔：
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簡(jiǎn)單介紹：
Recombinant Human Platelet Factor-4/CXCL4
詳情介紹：
    Synonyms
    Iroplact, Oncostatin-A
    Accession
    P02776
    GeneID
    5196
    Source
    Escherichia coli.
    Molecular Weight
    Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.
    AA Sequence
    EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES
    Purity
    > 97 % by SDS-PAGE and HPLC analyses.
    Biological Activity
    Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human fibroblasts is in a concentration of 1.0-10 ng/ml.
    Physical Appearance
    Sterile Filtered White lyophilized (freeze-dried) powder.
    Formulation
    Lyophilized from a 0.2 μm filtered concentrated solution in Acetonitrile and TFA.
    Endotoxin
    Less than 1 EU/μg of rHuPF-4/CXCL4 as determined by LAL method.
    Reconstitution
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
    Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    - 12 months from date of receipt, -20 to -70 °C as supplied.
    - 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
    Usage

    This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

      SDS-PAGE
      Background
      Platelet Factor-4 (PF-4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The gene for human CXCL4 is located on human chromosome 4. This chemokine is released from alpha-granules of activated platelets during platelet aggregation and neutralizes the anticoagulant effect of heparin. It interacts with a splice variant of the chemokine receptor CXCR3 besides be chemotactic for neutrophils, fibroblasts and monocytes. Recombinant human CXCL4 contains 70 amino acids binds with high affinity to heparin. Specifically, Human and mouse CXCL4 share about a 60 % identity.

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